Retroviruses pseudotyped with the severe acute respiratory syndrome coronavirus spike protein efficiently infect cells expressing angiotensin-converting enzyme 2
Identifieur interne : 005A74 ( Main/Exploration ); précédent : 005A73; suivant : 005A75Retroviruses pseudotyped with the severe acute respiratory syndrome coronavirus spike protein efficiently infect cells expressing angiotensin-converting enzyme 2
Auteurs : Michael J. Moore [États-Unis] ; Tatyana Dorfman [États-Unis] ; WENHUI LI [États-Unis] ; SWEE KEE WONG [États-Unis] ; YANHAN LI [République populaire de Chine] ; Jens H. Kuhn [États-Unis, Allemagne] ; James Coderre [États-Unis] ; Natalya Vasilieva [États-Unis] ; ZHONGCHAO HAN [République populaire de Chine] ; Thomas C. Greenough [États-Unis] ; Michael Farzan [États-Unis] ; HYERYUN CHOE [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Carboxypeptidases (génétique), Carboxypeptidases (métabolisme), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (métabolisme), Réplication virale, Séquence d'acides aminés, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), Virion (), Virion (métabolisme), Virus de l'immunodéficience simienne (génétique), Virus de l'immunodéficience simienne (métabolisme), Virus de l'immunodéficience simienne (physiologie), Virus de la leucémie murine (génétique), Virus de la leucémie murine (métabolisme), Virus de la leucémie murine (physiologie), Virus du SRAS (génétique).
- MESH :
- génétique : Carboxypeptidases, Glycoprotéines membranaires, Protéines de l'enveloppe virale, VIH-1 (Virus de l'Immunodéficience Humaine de type 1), Virus de l'immunodéficience simienne, Virus de la leucémie murine, Virus du SRAS.
- métabolisme : Carboxypeptidases, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Récepteurs viraux, Virion, Virus de l'immunodéficience simienne, Virus de la leucémie murine.
- physiologie : Virus de l'immunodéficience simienne, Virus de la leucémie murine.
- Pascal (Inist)
- Animaux, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, Retroviridae, Coronavirus, Grave, Malade état grave, Aigu, Réplication virale, Séquence d'acides aminés, Virion, Voie respiratoire, Protéine, Cellule infectée, Angiotensine, Peptidyl-dipeptidase A, Syndrome respiratoire aigu sévère, Microbiologie, Virologie, Forme grave.
English descriptors
- KwdEn :
- Acute, Amino Acid Sequence, Angiotensin, Animals, Carboxypeptidases (genetics), Carboxypeptidases (metabolism), Cell Line, Coronavirus, Critically ill, HIV-1 (genetics), Humans, Infected cell, Leukemia Virus, Murine (genetics), Leukemia Virus, Murine (metabolism), Leukemia Virus, Murine (physiology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Microbiology, Molecular Sequence Data, Peptidyl-Dipeptidase A, Peptidyl-dipeptidase A, Protein, Receptors, Virus (metabolism), Respiratory tract, Retroviridae, SARS Virus (genetics), Severe, Severe acute respiratory syndrome, Simian Immunodeficiency Virus (genetics), Simian Immunodeficiency Virus (metabolism), Simian Immunodeficiency Virus (physiology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Virion (chemistry), Virion (metabolism), Virology, Virus Replication.
- MESH :
- chemical , genetics : Carboxypeptidases, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Carboxypeptidases, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- chemistry : Virion.
- genetics : HIV-1, Leukemia Virus, Murine, SARS Virus, Simian Immunodeficiency Virus.
- metabolism : Leukemia Virus, Murine, Simian Immunodeficiency Virus, Virion.
- physiology : Leukemia Virus, Murine, Simian Immunodeficiency Virus.
- Amino Acid Sequence, Animals, Cell Line, Humans, Molecular Sequence Data, Peptidyl-Dipeptidase A, Spike Glycoprotein, Coronavirus, Virus Replication.
Abstract
Infection of receptor-bearing cells by coronaviruses is mediated by their spike (S) proteins. The coronavirus (SARS-CoV) that causes severe acute respiratory syndrome (SARS) infects cells expressing the receptor angiotensin-converting enzyme 2 (ACE2). Here we show that codon optimization of the SARS-CoV S-protein gene substantially enhanced S-protein expression. We also found that two retroviruses, simian immunodeficiency virus (SIV) and murine leukemia virus, both expressing green fluorescent protein and pseudotyped with SARS-CoV S protein or S-protein variants, efficiently infected HEK293T cells stably expressing ACE2. Infection mediated by an S-protein variant whose cytoplasmic domain had been truncated and altered to include a fragment of the cytoplasmic tail of the human immunodeficiency virus type 1 envelope glycoprotein was, in both cases, substantially more efficient than that mediated by wild-type S protein. Using S-protein-pseudotyped SIV, we found that the enzymatic activity of ACE2 made no contribution to S-protein-mediated infection. Finally, we show that a soluble and catalytically inactive form of ACE2 potently blocked infection by S-protein-pseudotyped retrovirus and by SARS-CoV. These results permit studies of SARS-CoV entry inhibitors without the use of live virus and suggest a candidate therapy for SARS.
Affiliations:
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Le document en format XML
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<s3>CHN</s3>
<sZ>5 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Tianjin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Greenough, Thomas C" sort="Greenough, Thomas C" uniqKey="Greenough T" first="Thomas C." last="Greenough">Thomas C. Greenough</name>
<affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Program in Molecular Medicine, University of Massachusetts Medical School</s1>
<s2>Worcester, Massachusetts</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Farzan, Michael" sort="Farzan, Michael" uniqKey="Farzan M" first="Michael" last="Farzan">Michael Farzan</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Partners AIDS Research Center, Brigham and Women's Hospital, and Department of Medicine (Microbiology and Molecular Genetics), and Perlmutter Laboratory, Children's Hospital</s1>
<s2>Boston</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hyeryun Choe" sort="Hyeryun Choe" uniqKey="Hyeryun Choe" last="Hyeryun Choe">HYERYUN CHOE</name>
<affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Department of Pediatrics, Harvard Medical School</s1>
<s2>Boston</s2>
<s3>USA</s3>
<sZ>8 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><settlement type="city">Boston</settlement>
<region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of virology</title>
<title level="j" type="abbreviated">J. virol.</title>
<idno type="ISSN">0022-538X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term>
<term>Amino Acid Sequence</term>
<term>Angiotensin</term>
<term>Animals</term>
<term>Carboxypeptidases (genetics)</term>
<term>Carboxypeptidases (metabolism)</term>
<term>Cell Line</term>
<term>Coronavirus</term>
<term>Critically ill</term>
<term>HIV-1 (genetics)</term>
<term>Humans</term>
<term>Infected cell</term>
<term>Leukemia Virus, Murine (genetics)</term>
<term>Leukemia Virus, Murine (metabolism)</term>
<term>Leukemia Virus, Murine (physiology)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Microbiology</term>
<term>Molecular Sequence Data</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Peptidyl-dipeptidase A</term>
<term>Protein</term>
<term>Receptors, Virus (metabolism)</term>
<term>Respiratory tract</term>
<term>Retroviridae</term>
<term>SARS Virus (genetics)</term>
<term>Severe</term>
<term>Severe acute respiratory syndrome</term>
<term>Simian Immunodeficiency Virus (genetics)</term>
<term>Simian Immunodeficiency Virus (metabolism)</term>
<term>Simian Immunodeficiency Virus (physiology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Virion (chemistry)</term>
<term>Virion (metabolism)</term>
<term>Virology</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Carboxypeptidases (génétique)</term>
<term>Carboxypeptidases (métabolisme)</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Récepteurs viraux (métabolisme)</term>
<term>Réplication virale</term>
<term>Séquence d'acides aminés</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term>
<term>Virion ()</term>
<term>Virion (métabolisme)</term>
<term>Virus de l'immunodéficience simienne (génétique)</term>
<term>Virus de l'immunodéficience simienne (métabolisme)</term>
<term>Virus de l'immunodéficience simienne (physiologie)</term>
<term>Virus de la leucémie murine (génétique)</term>
<term>Virus de la leucémie murine (métabolisme)</term>
<term>Virus de la leucémie murine (physiologie)</term>
<term>Virus du SRAS (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carboxypeptidases</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carboxypeptidases</term>
<term>Membrane Glycoproteins</term>
<term>Receptors, Virus</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term>
<term>Leukemia Virus, Murine</term>
<term>SARS Virus</term>
<term>Simian Immunodeficiency Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Carboxypeptidases</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term>
<term>Virus de l'immunodéficience simienne</term>
<term>Virus de la leucémie murine</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Leukemia Virus, Murine</term>
<term>Simian Immunodeficiency Virus</term>
<term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Carboxypeptidases</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Récepteurs viraux</term>
<term>Virion</term>
<term>Virus de l'immunodéficience simienne</term>
<term>Virus de la leucémie murine</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de l'immunodéficience simienne</term>
<term>Virus de la leucémie murine</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Leukemia Virus, Murine</term>
<term>Simian Immunodeficiency Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cell Line</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Retroviridae</term>
<term>Coronavirus</term>
<term>Grave</term>
<term>Malade état grave</term>
<term>Aigu</term>
<term>Réplication virale</term>
<term>Séquence d'acides aminés</term>
<term>Virion</term>
<term>Voie respiratoire</term>
<term>Protéine</term>
<term>Cellule infectée</term>
<term>Angiotensine</term>
<term>Peptidyl-dipeptidase A</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Microbiologie</term>
<term>Virologie</term>
<term>Forme grave</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Infection of receptor-bearing cells by coronaviruses is mediated by their spike (S) proteins. The coronavirus (SARS-CoV) that causes severe acute respiratory syndrome (SARS) infects cells expressing the receptor angiotensin-converting enzyme 2 (ACE2). Here we show that codon optimization of the SARS-CoV S-protein gene substantially enhanced S-protein expression. We also found that two retroviruses, simian immunodeficiency virus (SIV) and murine leukemia virus, both expressing green fluorescent protein and pseudotyped with SARS-CoV S protein or S-protein variants, efficiently infected HEK293T cells stably expressing ACE2. Infection mediated by an S-protein variant whose cytoplasmic domain had been truncated and altered to include a fragment of the cytoplasmic tail of the human immunodeficiency virus type 1 envelope glycoprotein was, in both cases, substantially more efficient than that mediated by wild-type S protein. Using S-protein-pseudotyped SIV, we found that the enzymatic activity of ACE2 made no contribution to S-protein-mediated infection. Finally, we show that a soluble and catalytically inactive form of ACE2 potently blocked infection by S-protein-pseudotyped retrovirus and by SARS-CoV. These results permit studies of SARS-CoV entry inhibitors without the use of live virus and suggest a candidate therapy for SARS.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Berlin</li>
<li>Massachusetts</li>
</region>
<settlement><li>Berlin</li>
<li>Boston</li>
<li>Tianjin</li>
</settlement>
</list>
<tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Moore, Michael J" sort="Moore, Michael J" uniqKey="Moore M" first="Michael J." last="Moore">Michael J. Moore</name>
</region>
<name sortKey="Coderre, James" sort="Coderre, James" uniqKey="Coderre J" first="James" last="Coderre">James Coderre</name>
<name sortKey="Dorfman, Tatyana" sort="Dorfman, Tatyana" uniqKey="Dorfman T" first="Tatyana" last="Dorfman">Tatyana Dorfman</name>
<name sortKey="Farzan, Michael" sort="Farzan, Michael" uniqKey="Farzan M" first="Michael" last="Farzan">Michael Farzan</name>
<name sortKey="Greenough, Thomas C" sort="Greenough, Thomas C" uniqKey="Greenough T" first="Thomas C." last="Greenough">Thomas C. Greenough</name>
<name sortKey="Hyeryun Choe" sort="Hyeryun Choe" uniqKey="Hyeryun Choe" last="Hyeryun Choe">HYERYUN CHOE</name>
<name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H." last="Kuhn">Jens H. Kuhn</name>
<name sortKey="Swee Kee Wong" sort="Swee Kee Wong" uniqKey="Swee Kee Wong" last="Swee Kee Wong">SWEE KEE WONG</name>
<name sortKey="Vasilieva, Natalya" sort="Vasilieva, Natalya" uniqKey="Vasilieva N" first="Natalya" last="Vasilieva">Natalya Vasilieva</name>
<name sortKey="Wenhui Li" sort="Wenhui Li" uniqKey="Wenhui Li" last="Wenhui Li">WENHUI LI</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Yanhan Li" sort="Yanhan Li" uniqKey="Yanhan Li" last="Yanhan Li">YANHAN LI</name>
</noRegion>
<name sortKey="Zhongchao Han" sort="Zhongchao Han" uniqKey="Zhongchao Han" last="Zhongchao Han">ZHONGCHAO HAN</name>
</country>
<country name="Allemagne"><region name="Berlin"><name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H." last="Kuhn">Jens H. Kuhn</name>
</region>
</country>
</tree>
</affiliations>
</record>
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